1. Princeton, Indiana, United States:
- The Toyota Highlander has been assembled at the Toyota Motor Manufacturing Indiana (TMMI) plant in Princeton, Indiana, since 2003.
2. Zhangjiagang, Jiangsu, China:
- Toyota also manufactures the Highlander at its joint venture facility with the Guangzhou Automobile Group (GAC) in Zhangjiagang, Jiangsu, China. This plant primarily contributes to meeting demand in the Chinese market.