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Can Flex fuel go into the 2007 Ford Expedition?

No, the 2007 Ford Expedition did not come standard with a flex-fuel engine. Therefore, you should not put flex fuel (E85) into it unless you have a specific flex-fuel vehicle (FFV) sticker on your engine bay or in your owner's manual clearly stating it's compatible. Using E85 in a non-FFV vehicle can cause significant engine damage.