No, a 2000 Ford Expedition transmission will almost certainly
not fit a 2000 Ford F-150, even if both are 2WD. While both are Ford trucks and might seem similar, the transmissions are different and not interchangeable. The bellhousing (where the transmission connects to the engine) and other mounting points are different. Even the bolt patterns will likely be incompatible.
You need to find a transmission specifically designed for the year and model of your F-150. The transmission code stamped on your F-150's transmission should be consulted when purchasing a replacement.