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Can you put a C-6 transmission in 1965 Ford 250 4x4 with straight 6 300?

No, you can't directly put a C6 automatic transmission in a 1965 Ford F250 4x4 with a straight-6 300 engine. The C6 was not designed to mate directly to that specific transfer case setup. The F250 4x4 of that era used a different transfer case and a different bellhousing pattern than what's compatible with the C6. You'd need significant modifications, including potentially a custom adapter plate or a completely different transmission and transfer case setup, which would be a very complex and expensive undertaking.