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Does a 1995 Chevy 3500 with 4L80E transmission have PTO?

No, a 1995 Chevy 3500 with a 4L80E transmission did not come standard with a PTO (Power Take-Off). PTOs were a factory option, and not a common one on these trucks. While it's *possible* a previous owner added one, it wasn't something included from the factory. You would have to inspect the truck to see if one has been installed.