The 2000 Ford F-250 5.4L Triton V8 engine's cylinder numbering follows a standard V8 pattern, typically viewed from the front of the engine. However, there's no single "cylinder 8." The cylinders are numbered sequentially, usually 1-8, with cylinder #1 being at the front passenger side and the numbering continuing in a "V" pattern.
Therefore, you'll need to specify which cylinder you are looking at or referring to. Cylinder 8 is located on the rear passenger side of the engine. But to be precise, you'd need a diagram or a visual inspection of the engine to be absolutely sure.