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Where is the fuel pump fuse in a 2001 F250 5.4L?

The fuel pump fuse location in a 2001 Ford F-250 with a 5.4L engine isn't a single fuse. Instead, the fuel pump relay controls the fuel pump. The relay itself isn't usually fused, but the circuit it's on *might* have a fuse protecting it (in addition to other fuses in the overall system).

You need to consult your owner's manual for the precise location of the fuel pump relay and any relevant fuses in the power distribution box (under the hood). The owner's manual will have a diagram showing fuse and relay locations.

Without the owner's manual, it's difficult to be certain, as the layout can vary slightly between trim levels. Searching online for "2001 Ford F250 5.4L fuse box diagram" might help you find a diagram to identify the relay and any associated fuses. Be sure to double-check that the diagram matches your specific truck's configuration.