1. Press the menu button on the Multi-function Display screen on your dashboard.
2. Select "Vehicle Settings" with the scroll button, then press the "Enter" button.
3. Use the scroll arrows to select "Seat Belts," and then press the "Enter" button.
4. Select "Unbuckle Warning," and choose the "Off" option.
The beep to fasten seat belts in your Lexus RX350 will be permanently disabled.