Will a 4l60e transmission work in 1990 1500 Chevy?

No, a 4L60E transmission will not directly work in a 1990 Chevy 1500. The 1990 model year used a different transmission, typically a TH700R4 (or possibly a 700R4). The 4L60E is a later electronically controlled transmission with different bolt patterns, control systems, and overall dimensions. You'd need significant modifications, likely including a custom driveshaft, different transmission mount, and rewiring, which is a complex and expensive undertaking. It's generally not a practical swap.